Journal: Breast Cancer Research and Treatment

Article Title: Targeting ataxia telangiectasia-mutated- and Rad3-related kinase (ATR) in PTEN-deficient breast cancers for personalized therapy

doi: 10.1007/s10549-018-4683-4

Figure Lengend Snippet: Association between PTEN, ATR and pChk1expression in breast cancers

Article Snippet: A further set of slides were incubated for 60 min with the primary rabbit polyclonal anti-phosphorylated Chk1 antibody (Ab58567, Abcam, Cambridge, UK), at a dilution of 1:140 [ ].

Techniques:

Journal: Breast Cancer Research and Treatment

Article Title: Targeting ataxia telangiectasia-mutated- and Rad3-related kinase (ATR) in PTEN-deficient breast cancers for personalized therapy

doi: 10.1007/s10549-018-4683-4

Figure Lengend Snippet: a Nuclear and cytoplasmic staining of PTEN [a negative staining for both, b negative staining for nuclei and weak for cytoplasm, c some weak staining for nuclei and moderate for cytoplasm d strong staining for both; TMA cores pictures were taken using digital pathology interference at ×100 (left) and ×200 (right)]. Kaplan–Meier plot showing breast cancer-specific survival (BCSS) and b nuclear PTEN level. c combined nuclear PTEN and ATR level. d combined nuclear PTEN and cytoplasmic pCHK1 level. e combined ATR and cytoplasmic pCHK1 level nuclear PTEN negative tumours

Article Snippet: A further set of slides were incubated for 60 min with the primary rabbit polyclonal anti-phosphorylated Chk1 antibody (Ab58567, Abcam, Cambridge, UK), at a dilution of 1:140 [ ].

Techniques: Staining, Negative Staining

Journal: Scientific Reports

Article Title: Salt suppresses IFNγ inducible chemokines through the IFNγ-JAK1-STAT1 signaling pathway in proximal tubular cells

doi: 10.1038/srep46580

Figure Lengend Snippet: The cells were treated by IFNγ (10 ng/mL) for 36 h after a 2 h exposure of culture medium supplemented with either NaCl (80 mM) or Sorbitol (160 mM, as an osmotic control). ( A ) Left, Representative immunoblotting performed to evaluate the phosphorylation of STAT1 in the nucleus; Right, Densitometry analysis of the immunoblotting of the phosphorylation of STAT1 in the nucleus (n = 5). Full-length western blot images are presented in . A high salt concentration inhibited phosphorylation of STAT1 at tyrosine 701 in the nucleus. ( B ) Left, Representative immunoblotting performed to evaluate the phosphorylation of JAK1 and JAK2; Right-upper, Densitometry analysis of the immunoblotting of the phosphorylation of JAK1 (n = 4); Right-lower, Densitometry analysis of the immunoblotting of the phosphorylation of JAK2 (n = 4). Full-length western blot images are presented in . A high salt concentration also inhibited phosphorylation of JAK1 at tyrosine 1022/1023, but not phosphorylation of JAK2 at tyrosine 1007/1008.

Article Snippet: Rabbit anti-STAT1 antibody, rabbit anti-phosphorylated STAT1 (Tyr 701 ) antibody, rabbit anti-JAK1 antibody, rabbit anti-phosphorylated JAK1 (Tyr 1022/1023 ) antibody, rabbit anti-JAK2 antibody, rabbit anti-phosphorylated JAK2 (Tyr 1007/1008 ) antibody, and rabbit anti-Histone H3 antibody were purchased from Cell Signaling.

Techniques: Control, Western Blot, Phospho-proteomics, Concentration Assay

Journal: Scientific Reports

Article Title: Salt suppresses IFNγ inducible chemokines through the IFNγ-JAK1-STAT1 signaling pathway in proximal tubular cells

doi: 10.1038/srep46580

Figure Lengend Snippet: The comparison between C57BL/6 mice fed a high salt diet for 7 days and mice fed a normal diet (n = 5). Left, Representative immunoblotting performed to evaluate the phosphorylation of JAK1 and JAK2 in kidney; Right-upper, Densitometry analysis of the immunoblotting of the phosphorylation of JAK1 (n = 4); Right-lower, Densitometry analysis of the immunoblotting of the phosphorylation of JAK2 (n = 4). Full-length western blot images are presented in . The phosphorylation of JAK1 at tyrosine 1022/1023 was inhibited in the kidney of C57BL/6 mice fed a high salt diet, whereas phosphorylation of JAK2 at tyrosine 1007/1008 was not inhibited.

Article Snippet: Rabbit anti-STAT1 antibody, rabbit anti-phosphorylated STAT1 (Tyr 701 ) antibody, rabbit anti-JAK1 antibody, rabbit anti-phosphorylated JAK1 (Tyr 1022/1023 ) antibody, rabbit anti-JAK2 antibody, rabbit anti-phosphorylated JAK2 (Tyr 1007/1008 ) antibody, and rabbit anti-Histone H3 antibody were purchased from Cell Signaling.

Techniques: Comparison, Western Blot, Phospho-proteomics

Journal: Scientific Reports

Article Title: Salt suppresses IFNγ inducible chemokines through the IFNγ-JAK1-STAT1 signaling pathway in proximal tubular cells

doi: 10.1038/srep46580

Figure Lengend Snippet: The cells were treated by a JAK1 and JAK2 specific inhibitor, ruxolitinib (Ruxo) (1 μM), 2 h before the 36 h of IFNγ (10 ng/mL) treatment. ( A ) Left, Representative immunoblotting performed to evaluate the phosphorylation of STAT1 in the nucleus; Right, Densitometry analysis of the immunoblotting of the phosphorylation of STAT1 in the nucleus (n = 4). Full-length western blot images are presented in . Ruxo inhibited phosphorylation of STAT1 at tyrosine 701 in the nucleus. ( B ) qRT-PCR analyses were performed to evaluate CXCLs expression (n = 4). The expressions of CXCLs were markedly suppressed and no difference between the high salt concentration condition and control condition was evident.

Article Snippet: Rabbit anti-STAT1 antibody, rabbit anti-phosphorylated STAT1 (Tyr 701 ) antibody, rabbit anti-JAK1 antibody, rabbit anti-phosphorylated JAK1 (Tyr 1022/1023 ) antibody, rabbit anti-JAK2 antibody, rabbit anti-phosphorylated JAK2 (Tyr 1007/1008 ) antibody, and rabbit anti-Histone H3 antibody were purchased from Cell Signaling.

Techniques: Western Blot, Phospho-proteomics, Quantitative RT-PCR, Expressing, Concentration Assay, Control

Journal: Frontiers in Pharmacology

Article Title: Exercise ameliorates hepatic lipid accumulation via upregulating serum PRL and activating hepatic PRLR-mediated JAK2/STAT5 signaling pathway in NAFLD mice

doi: 10.3389/fphar.2025.1647231

Figure Lengend Snippet: Expression of PRL/PRLR signaling pathway related proteins in liver tissue, and expression levels of PRLR (A) , JAK2 (B) , p-JAK2 (C) , STAT5a (D) , and p-STAT5 (E) in liver tissue of mice in each group.

Article Snippet: The PVDF membrane was blocked with 5% bovine serum albumin (BSA) at room temperature for 1 h and then incubated overnight at 4°C with gentle shaking in the presence of the following primary rabbit monoclonal antibodies: total PRLR (1:500 dilution, ER 1915-43, Huabio), JAK2 (1:1000 dilution, ET1607-35, Huabio), phosphorylated JAK2 (p-JAK2; 1:1000 dilution, ET1607-34, Huabio), STAT5a (1:1000 dilution, ET1610-58, Huabio), phosphorylated STAT5 (p-STAT5; 1:1000 dilution, ET1610-48, Huabio), and β-actin (1:100,000 dilution, AC026, Abclonal).

Techniques: Expressing